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Proteintech rabbit antibodies recognizing ythdf1

a RIP analyses of KYSE180 cells were performed with an <t>anti-YTHDF1</t> or anti-YTHDF2, or anti-YTHDF3 antibody followed by qPCR analyses with primers against APC mRNA. Data represent the means ± SD of triplicate samples. *** p = 0.0006 (left), ** p = 0.0092 (right) based on two-tailed Student’s t -test. b KYSE180 cells were transfected with or without a vector expressing METTL3 shRNA. RIP analyses were performed with an anti-YTHDF2 antibody followed by qPCR analyses with primers against APC mRNA. Data represent the means ± SD of triplicate samples. *** p = 1.85E − 05 (left), ** p = 0.0023 (middle) and 0.0056 (right) based on two-tailed Student’s t -test. c KYSE450 cells were transfected with or without a vector expressing YTHDF2 shRNA. The relative mRNA expression levels of APC were measured using quantitative PCR. Data represent the means ± SD of triplicate samples. *** p = 0.0006, 0.0009, 5.58E − 05 and 0.0007 (left to right) based on two-tailed Student’s t -test. d KYSE450 cells were transfected with or without YTHDF2 shRNA. Immunoblotting analyses were performed with the indicated antibodies for three times with similar results. e KYSE180 cells were transfected with or without a YTHDF2 siRNA or combination of YTHDF1–3 siRNAs. The relative mRNA expression levels of APC were measured using quantitative PCR. Data represent the means ± SD of triplicate samples. *** p = 9.33E − 04 (left) and 2.99E − 05 (right), * p = 0.031 based on two-tailed Student’s t -test. f KYSE180 cells were transfected with or without a YTHDF2 siRNA or combination of YTHDF1–3 siRNAs. Immunoblotting analyses were performed with the indicated antibodies for three times with similar results. g KYSE180 cells expressing luciferase reporter genes fused with or without the wild-type (WT) or mutated m6A nucleotides from APC genes were transfected with or without a vector expressing YTHDF2 shRNA. The relative luciferase activity after normalization to the shControl group is shown. Data represent the means ± SD of triplicate samples. *** p = 3.94E − 06 based on two-tailed Student’s t -test. ns, not significant. Source data are provided as a Source Data file.

Rabbit Antibodies Recognizing Ythdf1, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 401 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+antibodies+recognizing+ythdf1/YTHDF1+Antibody/pmc08217513-200-0-50
Average 96 stars, based on 401 article reviews

rabbit antibodies recognizing ythdf1 - by Bioz Stars, 2026-09

96/100 stars

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1) Product Images from "METTL3 promotes tumour development by decreasing APC expression mediated by APC mRNA N 6 -methyladenosine-dependent YTHDF binding"

Article Title: METTL3 promotes tumour development by decreasing APC expression mediated by APC mRNA N 6 -methyladenosine-dependent YTHDF binding

Journal: Nature Communications

doi: 10.1038/s41467-021-23501-5

a RIP analyses of KYSE180 cells were performed with an anti-YTHDF1 or anti-YTHDF2, or anti-YTHDF3 antibody followed by qPCR analyses with primers against APC mRNA. Data represent the means ± SD of triplicate samples. *** p = 0.0006 (left), ** p = 0.0092 (right) based on two-tailed Student’s t -test. b KYSE180 cells were transfected with or without a vector expressing METTL3 shRNA. RIP analyses were performed with an anti-YTHDF2 antibody followed by qPCR analyses with primers against APC mRNA. Data represent the means ± SD of triplicate samples. *** p = 1.85E − 05 (left), ** p = 0.0023 (middle) and 0.0056 (right) based on two-tailed Student’s t -test. c KYSE450 cells were transfected with or without a vector expressing YTHDF2 shRNA. The relative mRNA expression levels of APC were measured using quantitative PCR. Data represent the means ± SD of triplicate samples. *** p = 0.0006, 0.0009, 5.58E − 05 and 0.0007 (left to right) based on two-tailed Student’s t -test. d KYSE450 cells were transfected with or without YTHDF2 shRNA. Immunoblotting analyses were performed with the indicated antibodies for three times with similar results. e KYSE180 cells were transfected with or without a YTHDF2 siRNA or combination of YTHDF1–3 siRNAs. The relative mRNA expression levels of APC were measured using quantitative PCR. Data represent the means ± SD of triplicate samples. *** p = 9.33E − 04 (left) and 2.99E − 05 (right), * p = 0.031 based on two-tailed Student’s t -test. f KYSE180 cells were transfected with or without a YTHDF2 siRNA or combination of YTHDF1–3 siRNAs. Immunoblotting analyses were performed with the indicated antibodies for three times with similar results. g KYSE180 cells expressing luciferase reporter genes fused with or without the wild-type (WT) or mutated m6A nucleotides from APC genes were transfected with or without a vector expressing YTHDF2 shRNA. The relative luciferase activity after normalization to the shControl group is shown. Data represent the means ± SD of triplicate samples. *** p = 3.94E − 06 based on two-tailed Student’s t -test. ns, not significant. Source data are provided as a Source Data file.

Figure Legend Snippet: a RIP analyses of KYSE180 cells were performed with an anti-YTHDF1 or anti-YTHDF2, or anti-YTHDF3 antibody followed by qPCR analyses with primers against APC mRNA. Data represent the means ± SD of triplicate samples. *** p = 0.0006 (left), ** p = 0.0092 (right) based on two-tailed Student’s t -test. b KYSE180 cells were transfected with or without a vector expressing METTL3 shRNA. RIP analyses were performed with an anti-YTHDF2 antibody followed by qPCR analyses with primers against APC mRNA. Data represent the means ± SD of triplicate samples. *** p = 1.85E − 05 (left), ** p = 0.0023 (middle) and 0.0056 (right) based on two-tailed Student’s t -test. c KYSE450 cells were transfected with or without a vector expressing YTHDF2 shRNA. The relative mRNA expression levels of APC were measured using quantitative PCR. Data represent the means ± SD of triplicate samples. *** p = 0.0006, 0.0009, 5.58E − 05 and 0.0007 (left to right) based on two-tailed Student’s t -test. d KYSE450 cells were transfected with or without YTHDF2 shRNA. Immunoblotting analyses were performed with the indicated antibodies for three times with similar results. e KYSE180 cells were transfected with or without a YTHDF2 siRNA or combination of YTHDF1–3 siRNAs. The relative mRNA expression levels of APC were measured using quantitative PCR. Data represent the means ± SD of triplicate samples. *** p = 9.33E − 04 (left) and 2.99E − 05 (right), * p = 0.031 based on two-tailed Student’s t -test. f KYSE180 cells were transfected with or without a YTHDF2 siRNA or combination of YTHDF1–3 siRNAs. Immunoblotting analyses were performed with the indicated antibodies for three times with similar results. g KYSE180 cells expressing luciferase reporter genes fused with or without the wild-type (WT) or mutated m6A nucleotides from APC genes were transfected with or without a vector expressing YTHDF2 shRNA. The relative luciferase activity after normalization to the shControl group is shown. Data represent the means ± SD of triplicate samples. *** p = 3.94E − 06 based on two-tailed Student’s t -test. ns, not significant. Source data are provided as a Source Data file.

Techniques Used: Two Tailed Test, Transfection, Plasmid Preparation, Expressing, shRNA, Real-time Polymerase Chain Reaction, Western Blot, Luciferase, Activity Assay

Western Blot:

Article Title: METTL3 promotes tumour development by decreasing APC expression mediated by APC mRNA N 6 -methyladenosine-dependent YTHDF binding
Article Snippet: A rabbit antibody recognizing METTL3 (ab195352) (for IHC, 1 : 50 dilution and RIP), APC (ab15270) (for IHC, 1 : 100 dilution), APC (ab154906) (for immunoblotting, 1 : 1000 dilution) and c-Myc (ab32072, 1 : 1000 dilution) (for immunoblotting, 1 : 50 dilution) was purchased from Abcam (Cambridge, MA). .. Rabbit antibodies recognizing YTHDF1 (17479-1-AP) (for RIP, 1 : 50 dilution and immunoblotting, 1 : 1000 dilution) and YTHDF2 (24744-1-AP) (for RIP, 1 : 50 dilution and immunoblotting, 1 : 1000 dilution) and YTHDF3 (25537-1-AP) (for RIP, 1 : 50 dilution and immunoblotting, 1 : 1000 dilution) were purchased from Proteintech (IL, USA). .. The anti-m 6 A polyclonal antibody was purchased from Synaptic Systems.

Image Search Results

Journal: Nature Communications

Article Title: METTL3 promotes tumour development by decreasing APC expression mediated by APC mRNA N 6 -methyladenosine-dependent YTHDF binding

doi: 10.1038/s41467-021-23501-5

Figure Lengend Snippet: a RIP analyses of KYSE180 cells were performed with an anti-YTHDF1 or anti-YTHDF2, or anti-YTHDF3 antibody followed by qPCR analyses with primers against APC mRNA. Data represent the means ± SD of triplicate samples. *** p = 0.0006 (left), ** p = 0.0092 (right) based on two-tailed Student’s t -test. b KYSE180 cells were transfected with or without a vector expressing METTL3 shRNA. RIP analyses were performed with an anti-YTHDF2 antibody followed by qPCR analyses with primers against APC mRNA. Data represent the means ± SD of triplicate samples. *** p = 1.85E − 05 (left), ** p = 0.0023 (middle) and 0.0056 (right) based on two-tailed Student’s t -test. c KYSE450 cells were transfected with or without a vector expressing YTHDF2 shRNA. The relative mRNA expression levels of APC were measured using quantitative PCR. Data represent the means ± SD of triplicate samples. *** p = 0.0006, 0.0009, 5.58E − 05 and 0.0007 (left to right) based on two-tailed Student’s t -test. d KYSE450 cells were transfected with or without YTHDF2 shRNA. Immunoblotting analyses were performed with the indicated antibodies for three times with similar results. e KYSE180 cells were transfected with or without a YTHDF2 siRNA or combination of YTHDF1–3 siRNAs. The relative mRNA expression levels of APC were measured using quantitative PCR. Data represent the means ± SD of triplicate samples. *** p = 9.33E − 04 (left) and 2.99E − 05 (right), * p = 0.031 based on two-tailed Student’s t -test. f KYSE180 cells were transfected with or without a YTHDF2 siRNA or combination of YTHDF1–3 siRNAs. Immunoblotting analyses were performed with the indicated antibodies for three times with similar results. g KYSE180 cells expressing luciferase reporter genes fused with or without the wild-type (WT) or mutated m6A nucleotides from APC genes were transfected with or without a vector expressing YTHDF2 shRNA. The relative luciferase activity after normalization to the shControl group is shown. Data represent the means ± SD of triplicate samples. *** p = 3.94E − 06 based on two-tailed Student’s t -test. ns, not significant. Source data are provided as a Source Data file.

Article Snippet: Rabbit antibodies recognizing YTHDF1 (17479-1-AP) (for RIP, 1 : 50 dilution and immunoblotting, 1 : 1000 dilution) and YTHDF2 (24744-1-AP) (for RIP, 1 : 50 dilution and immunoblotting, 1 : 1000 dilution) and YTHDF3 (25537-1-AP) (for RIP, 1 : 50 dilution and immunoblotting, 1 : 1000 dilution) were purchased from Proteintech (IL, USA).

Techniques: Two Tailed Test, Transfection, Plasmid Preparation, Expressing, shRNA, Real-time Polymerase Chain Reaction, Western Blot, Luciferase, Activity Assay

A MYC mRNA level in different pools of ribosomes from H322 cells with or without FTO depletion was determined by polysome profiling and qPCR assays. Data represent the means ± SD of triplicate samples. * P < 0.05, ** P < 0.01. B RIP analyses of H322 cells were performed with an anti-YTHDF1 antibody followed by qPCR analyses with primers against MYC mRNA. Data represent the means ± SD of triplicate samples. **** P < 0.0001. C qPCR analysis was performed for mRNA levels of YTHDF1 and MYC in H322 cells with or without YTHDF1 depletion. Data represent the means ± SD of triplicate samples. ** P < 0.01. D H322 cells with or without YTHDF1 depletion were analyzed by immunoblotting assays with the indicated antibodies. E YTHDF1 shRNA was expressed in H358 cells with or without FTO depletion. Immunoblotting analyses were performed with the indicated antibodies.

Journal: Cell Death & Disease

Article Title: WNT/β-catenin-suppressed FTO expression increases m 6 A of c-Myc mRNA to promote tumor cell glycolysis and tumorigenesis

doi: 10.1038/s41419-021-03739-z

Figure Lengend Snippet: A MYC mRNA level in different pools of ribosomes from H322 cells with or without FTO depletion was determined by polysome profiling and qPCR assays. Data represent the means ± SD of triplicate samples. * P < 0.05, ** P < 0.01. B RIP analyses of H322 cells were performed with an anti-YTHDF1 antibody followed by qPCR analyses with primers against MYC mRNA. Data represent the means ± SD of triplicate samples. **** P < 0.0001. C qPCR analysis was performed for mRNA levels of YTHDF1 and MYC in H322 cells with or without YTHDF1 depletion. Data represent the means ± SD of triplicate samples. ** P < 0.01. D H322 cells with or without YTHDF1 depletion were analyzed by immunoblotting assays with the indicated antibodies. E YTHDF1 shRNA was expressed in H358 cells with or without FTO depletion. Immunoblotting analyses were performed with the indicated antibodies.

Article Snippet: Rabbit antibody recognizing YTHDF1 (17479-1-AP) (for RIP and immunoblotting) was purchased from Proteintech (IL, USA).

Techniques: Western Blot, shRNA